Reconstructing cell histories in space with image-readable base editor recording
<p>The paper introduces a new approach called “baseMEMOIR” for recording detailed cell lineage information while maintaining spatial context.</p>
<p>BaseMEMOIR uses a CRISPR base editor to introduce stochastic edits at engineered dinucleotide sequences distributed across the genome. Each dinucleotide can be edited to one of three states, allowing diverse barcode recording. The authors integrated many copies of an editable barcode array into mouse embryonic stem cells, alongside inducible base editor and guide RNA expression systems. This provided hundreds of bits of recordable information per cell.</p>
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