Clustered regularly interspaced palindromic repeats (CRISPR) is a gene editing technology, that makes it possible to correct errors in the genome and manipulate the genes with relative ease. It has applications in the live imaging of the genome and also in generation of cellular models. It has been used to repair defective DNA in mice to correct the genetic errors. It is currently used in genome silencing and knock-in experiments as well as transcriptional activation and repression. It has 2 components: a guide RNA (gRNA) and a Cas9 nuclease, which has been conferred below. The clinical applications, along with the limitations and ethical considerations will also be discussed.
Why Scrum Fails
This ubiquitous thing called “Scrum” is an oppressive tool for micromanagement. It’s all about story points and velocity. It has earned the utter contempt of developers, designers, product managers, and middle managers alike. The maddening…