SynBio — p.20: A Plasmid Design-driven Directed Evolution Approach to Engineer Enzymes

<p>Directed evolution is an innovative technique that emulates the natural selection process to engineer enzymes with improved properties, such as increased activity, specificity, or stability. The method involves three steps: a) subjecting a gene to iterative cycles of mutagenesis (generating a variant library), b) selection (expressing and isolating variants with desired traits), and c) amplification (creating a template for subsequent rounds). Directed Evolution can be conducted either in vivo (within living organisms) or in vitro (in cells or solution) and serves as an alternative approach to the rational design of modified proteins. It essentially facilitates experimental evolution studies of fundamental evolutionary principles in a controlled laboratory setting.</p> <p><a href="https://medium.com/@piasaha.io/synbio-p-20-a-plasmid-design-driven-directed-evolution-approach-to-engineer-enzymes-6ac5d0aa69c9"><strong>Click Here</strong></a></p>