Genetic Engineering: designing a sgRNA using Benchling and CRISPR Cas9 Gene Editing Technology

<p>In this article, I am going to explain&nbsp;<strong>genetic engineering</strong>&nbsp;and introduce&nbsp;<strong>gene editing</strong>&nbsp;for medical applications. I will then, describe&nbsp;<strong>the working of CRISPR</strong>, which is the next big thing when it comes to genome editing technology. You will want to stick around till the end of this article: I<strong>&nbsp;designed a sgRNA using Benchling</strong>&nbsp;(a platform for biotechnology research) and CRISPR Cas9 Gene Editing Technology. I made a tutorial for it, which you can replicate to better understand the workings of CRISPR.</p> <h1>Genetic engineering</h1> <p>Genetic engineering depends on modifying the 4 building blocks of life, the&nbsp;<strong>4 nucleotides.</strong>&nbsp;These are found in DNA and are&nbsp;<strong>adenine (A), thymine (T), cytosine (C), and guanine (G)</strong>. They are also each made up of a nitrogenous base, a deoxyribose sugar, and a phosphate group. Adenine pairs with thymine, and cytosine pairs with guanine in the DNA double helix through specific hydrogen bonding.</p> <p><a href="https://medium.com/@davidoula.mg/genetic-engineering-designing-a-sgrna-using-benchling-and-crispr-cas9-gene-editing-technology-4b56fba98831"><strong>Website</strong></a></p>