What is the Difference between C8 and C18 column in HPLC?

<p>C8 column is a type of column used in reverse-phase chromatography. Generally, reverse-phase chromatography uses a hydrophobic stationary phase. However, the solid support the stationary phase or the column contains modified silica. Here, silica is a hydrophilic stationary phase.</p> <p>C8 column is a form of column present in some HPLC apparatuses, and it has Octylsilane as its stationary phase. And, this compound in the stationary phase has 8 carbon atoms in its alkyl chain. Further, it tends to retain components of the analyte less than that of the C18 column. Therefore, a compound will elute faster in a C8 column.</p> <p>However, it is less dense because it has a less number of carbon atoms; the length of the carbon chain of this compound in the stationary phase is short. Moreover, nonpolar compounds move down the column rapidly with the C8 column. It is mainly because of the lower hydrophobicity of the C8 compound.</p> <p><a href="https://medium.com/@patel.mubarak2/what-is-the-difference-between-c8-and-c18-column-in-hplc-2aae8f3b1211"><strong>Click Here</strong></a></p>