White Paper: Imaging-based viability assessment of cytostatic vs cytosolic chemotherapies in 3D culture using a novel high-throughput multi-camera array microscope

CellTiter-Glo (CTG) is the gold standard for assessment of cellular viability due to its ease of use and fast turnaround time for data analysis. Multiple commercially available systems are capable of assessing imaging-based viability, but the speed of acquisition and time-consuming analysis limits the sample throughput compared to CTG. Additionally, without an automated work cell to feed plates back and forth between the incubator and the imaging system, a researcher must spend a significant amount of hands-on time to complete the workflow. The Multi-Camera Array Microscope (MCAM™) by Ramona Optics offers unprecedented speed with its high-throughput imaging technology and cutting-edge data analysis tools. The MCAM™ can compete with the speed of acquisition and data analysis of a plate reader but offers a higher content of data gained by imaging. Imaging-based viability assessment offers the ability to collect data on a cellular level as opposed to the per-well data offered from plate readers. Cellular-level data provides information around heterogeneity of cellular toxicity responses to various treatment conditions, which is of great significance to in vitro heterogeneous disease models, such as cancer. Additionally, an imaging-based approach enables researchers to distinguish between drug cytotoxic and cytostatic mechanisms of action which is not possible with traditional CTG assays. <a href="https://medium.com/ramonaoptics/white-paper-imaging-based-viability-assessment-of-cytostatic-vs-cytosolic-chemotherapies-in-3d-614958b74464">Website</a>