Genetically encoded barcodes for correlative volume electron microscopy

<p>The paper introduces a new method for genetically encoding electron microscopy (EM) contrast agents called EMcapsulins. EMcapsulins are based on self-assembling protein nanocompartments called encapsulins.</p> <p>By fusing different numbers (1&ndash;3) of metallothionein domains to the inner surface of encapsulins of different sizes, the authors generated 6 classes of circular barcodes that can be differentiated in EM images. The barcoded EMcapsulins provide nanometer-scale contrast that is detectable by standard EM sample preparation and imaging techniques, without needing extra steps like substrate incubation.</p> <p><a href="https://medium.com/@axialxyz/genetically-encoded-barcodes-for-correlative-volume-electron-microscopy-fbe76953c55e"><strong>Click Here</strong></a></p>