3 major tools & techniques used for Plant Tissue Culture
<p>An autoclave is used to sterilize medium, glassware, and tools for plant tissue culture. Sterilization is carried out by increasing moist heat (121 degrees Celsius) due to increased pressure inside the vessel (15–22 psi, pounds per square inch or 1.02 to 1.56 kg/cm2)for 15 minutes for routine sterilization. Moist heat kills the microorganism and makes the material contaminate free from microbes.</p>
<p>The autoclave may be constructed of aluminum, mild steel, or gunmetal. Industrial autoclaves can accommodate large trolleys containing large numbers of glassware or large bioreactors.</p>
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