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CRISPR/Cas9: A review

Clustered regularly interspaced palindromic repeats (CRISPR) is a gene editing technology, that makes it possible to correct errors in the genome and manipulate the genes with relative ease. It has applications in the live imaging of the genome and also in generation of cellular models. It has been used to repair defective DNA in mice to correct the genetic errors. It is currently used in genome silencing and knock-in experiments as well as transcriptional activation and repression. It has 2 components: a guide RNA (gRNA) and a Cas9 nuclease, which has been conferred below. The clinical applications, along with the limitations and ethical considerations will also be discussed.

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