An Fc engineering approach that modulates antibody-dependent cytokine release without altering cell-killing functions

<p>This research article investigates an Fc engineering approach for monoclonal antibodies (mAbs) that modulates antibody-dependent cytokine release (ADCR) without altering cell-killing functions. IgG1 mAbs elicited robust ADCC (antibody-dependent cell-mediated cytotoxicity) and ADCR of proinflammatory cytokines like IFN&gamma;, IL-1&alpha;, and IL-1&beta; from peripheral blood mononuclear cells (PBMCs). In contrast, IgG2 and IgG4 mAbs had limited ability to elicit these responses.</p> <p>Macrophage-mediated ADCP (antibody-dependent cellular phagocytosis) was elicited by IgG1, IgG2 and IgG4 mAbs. However, IgG1 and IgG4 also induced high levels of anti-inflammatory cytokine IL-10 and other cytokines like IL-8, MIP-1&alpha;, MIP-1&beta;, and TNF. IgG2 induced ADCP but minimal cytokine release.</p> <p><a href="https://medium.com/@axialxyz/an-fc-engineering-approach-that-modulates-antibody-dependent-cytokine-release-without-altering-70ef9597ffa1"><strong>Learn More</strong></a></p>